UJI AKTIVITAS ANTIBAKTERI PADA MACAM MACAM MADU PADA BAKTERI ESCHERICHIA COLI DAN STAPHYLOCOCCUS AUREUS DENGAN METODE DIFUSI AGAR DAN DILUSI CAIR

Siti Aisyah Ratna  Putri; Haryanto Susanto; Sabrina Handayani Tambun; Teguh  Oktiarso

doi:10.33479/sb.v2i2.153

Authors

Siti Aisyah Ratna Putri Mahasiswa Farmasi, Fakultas Sains & Teknologi, Universitas Ma Chung
Haryanto Susanto Dosen Farmasi, Fakultas Sains & Teknologi, Universitas Ma Chung
Sabrina Handayani Tambun Dosen Farmasi, Fakultas Sains & Teknologi, Universitas Ma Chung
Teguh Oktiarso Dosen Teknik Industri, Fakultas Sains & Teknologi, Universitas Ma Chung

DOI:

https://doi.org/10.33479/sb.v2i2.153

Keywords:

antibakteri, madu, KHM, KBM, E.coli, S.aureus, difusi cakram, dilusi

Abstract

This study is an experimental laboratory, conducted to get the antibacterial activity in the types of multiflora honey, randu honey, mente honey, rambutan honey, sono honey, acacia honey, rubber honey. In this study, preliminary tests, phytochemical screening, and antibacterial tests have been carrying out to determine the Minimum Inhibitory Level (MIC) and Minimum Killing Rate (KBM) at concentrations of 20, 25, 50, and 100%. Phytochemical test results it's known that each honey contains positive alkaloids, flavonoids, glycosides, and saponins. Antibacterial test results showed that each honey's positively had antibacterial activity. MIC and KBM obtained showed that each honey can inhibit the growth of bacteria, but only rambutan honey in Escherichia coli bacteria cannot kill bacteria.

Keywords: antibacterial, honey, MIC, KBM, E.coli, S.aureus, disc diffusion, dilution